Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Plasma membrane, Cytoplasm

Additional location(s)

 

Staining summary

Localized to nucleus, cytoplasm and plasma membrane.

Reliability (APE)

Medium

Antibodies in assay

HPA019044, CAB022157, CAB025216
 

Antibody HPA019044

 

Antibody CAB022157

 

Antibody CAB025216

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm Nucleus, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

Granular, Spotty Spotty  

Staining intensity

Moderate Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus, Plasma membrane, Cytoplasm Nucleus, Nucleoli, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

Granular, Spotty Cluster  

Staining intensity

Moderate Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm Nucleus but not nucleoli, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

Granular, Spotty Spotty  

Staining intensity

Weak Strong Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of nucleus, plasma membrane and cytoplasm in all three cell lines.   Staining of nucleus and cytoplasm in all three cell lines with additional staining of nucleoli in U-2 OS.   Staining of plasma membrane and cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus
Plasma membrane