Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus but not nucleoli 

Staining summary

Mainly localized to the cytoplasm. In addition localized to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA006148, CAB017441
 

Antibody HPA006148

 

Antibody CAB017441

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm Cytoplasm 

Staining characteristics

  

Staining intensity

Strong Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm   

Staining characteristics

   

Staining intensity

Strong   

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
   

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of nucleus in U-251 MG and A-431.   Staining of cytoplasm in A-431 and U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli