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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane

Additional location(s)

Cytoplasm, Cytoskeleton (Actin filaments) 

Staining summary

The protein is localized to the plasma membrane, cytoplasm and actin filaments in the three cell lines.

Reliability (APE)

Low

Antibodies in assay

HPA030117, HPA030118
 

Antibody HPA030117

 

Antibody HPA030118

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments)  

Staining characteristics

  

Staining intensity

Moderate Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments)  

Staining characteristics

  

Staining intensity

Moderate Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments)  

Staining characteristics

  

Staining intensity

Strong Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane, cytoplasm & cytoskeleton (actin filaments).
 

Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Summary

Staining of plasma membrane, cytoplasm and cytoskeleton (actin filaments) in all three cell lines.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Actin filaments
Plasma membrane