WDR54

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA043257

 

Antibody HPA053558

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA043257 HPA053558 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

8 12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

77 86 

Antigen sequence

RVLVFDIPAKGPNIVLSEELAGHQMPITDIATEPAQGQDCVADMVTADDS
GLLCVWRSGPEFTLLTRIPGFGVPCPS
 
TTGNLHVQINAHARAICALDLASEVGKLLSAGEDTFVHIWKLSRNPESGY
IEVEHCHGECVADTQLCGARFCDSSGNSFAVTGYDL
 

Matching transcripts

WDR54-001 - ENSP00000006526 [100%]
WDR54-005 - ENSP00000387236 [100%]
 WDR54-001 - ENSP00000006526 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
More information
  

Retrieval method

HIER pH6  

Antibody dilution

1:200  

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

35.9, 26.2 35.9 

Antibody dilution

1:250 1:390 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3150 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 

         Immunofluorescence

Image

  

Description

 Immunofluorescent staining of human cell line MCF-7 shows positivity in mitochondria & vesicles.
More information
 

Antibody dilution

 1:78 

Validation IF

 Uncertain: The subcellular location is partly supported by literature or no literature is available. 
 

ANTIGEN VIEW

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WDR54-001
 
WDR54-005