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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Mitochondria

Additional location(s)

Cytoplasm 

Staining summary

Staining of nucleus in all three cell lines. Additional staining of mitochondria in U-2 OS, cytoplasm in U-251 MG and both mitochondria and cytoplasm in A-431.

Reliability (Single)

Antibodies in assay

HPA008287
 

Antibody HPA008287

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Mitochondria 

Staining characteristics

Smooth 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Mitochondria 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, cytoplasm & mitochondria.
 

Summary

Staining of nucleus in all three cell lines. Additional staining of mitochondria in U-2 OS, cytoplasm in U-251 MG and both mitochondria and cytoplasm in A-431.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Mitochondria
Nucleus but not nucleoli