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SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm, Golgi apparatus

Additional location(s)

Nucleus but not nucleoli, Plasma membrane 

Staining summary

Staining of cytoplasm and Golgi in all three cell lines with additional staining of plasma membrane in A-431 and nucleus in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA014429
 

Antibody HPA014429

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm, Golgi apparatus 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Golgi apparatus 

Staining characteristics

Granular 

Staining intensity

Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Cytoplasm, Golgi apparatus 

Staining characteristics

Granular 

Staining intensity

Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm & golgi apparatus.
 

Summary

Staining of cytoplasm and Golgi in all three cell lines with additional staining of plasma membrane in A-431 and nucleus in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Golgi apparatus
Nucleus but not nucleoli
Plasma membrane