Fields »

SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane

Additional location(s)

Cytoplasm 

Staining summary

Mainly localized to the plasma membrane but also to the cytoplasm.

Reliability (APE)

High

Antibodies in assay

HPA019484, HPA021257, HPA023874
 

Antibody HPA019484

 

Antibody HPA021257

 

Antibody HPA023874

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm, Cell Junctions Plasma membrane, Cytoplasm 

Staining characteristics

Granular  Granular 

Staining intensity

Weak Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

  Granular 

Staining intensity

Moderate Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

 Granular Granular 

Staining intensity

Weak Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of nucleus in U-251 MG and plasma membrane in A-431 and U-2 OS.   Staining of plasma membrane and cytoplasm in all three cell lines with additional staining of cell junctions in A-431.   Staining of plasma membrane and cytoplasm in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Plasma membrane