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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Cytoplasm, Golgi apparatus, Vesicles 

Staining summary

Mainly localized to the nucleus but also to the Golgi apparatus, vesicles and the cytoplasm.

Reliability (APE)

Low

Antibodies in assay

HPA007446, HPA027751
 

Antibody HPA007446

 

Antibody HPA027751

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Golgi apparatus Nucleus but not nucleoli, Vesicles 

Staining characteristics

Granular  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Vesicles 

Staining characteristics

Granular  

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Golgi apparatus Nucleus but not nucleoli, Vesicles 

Staining characteristics

Granular Granular 

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli, cytoplasm & golgi apparatus.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & vesicles.
 

Summary

Staining of nucleus and cytoplasm in all three cell lines with additional staining of Golgi apparatus in U-251MG and A-431.   Staining of nucleus and vesicles in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Golgi apparatus
Nucleus but not nucleoli
Endosome