Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

 

Staining summary

Localized to the nucleus.

Reliability (APE)

High

Antibodies in assay

HPA002847, CAB011577
 

Antibody HPA002847

 

Antibody CAB011577

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

Smooth  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm Nucleus but not nucleoli 

Staining characteristics

Granular  

Staining intensity

Moderate Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nucleus in U-251 MG and A-431 and cytoplasm in U-2 OS.   Staining of the nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleus but not nucleoli