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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

Cytoskeleton (Microtubules) 

Staining summary

Staining of cytoplasm and nucleus in the three cell lines with additional staining of cytoskeleton (microtubules) in U-251 MG and U-2 OS.

Reliability (Single)

Antibodies in assay

HPA023189
 

Antibody HPA023189

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Cytoskeleton (Microtubules) 

Staining characteristics

Granular 

Staining intensity

Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Cytoskeleton (Microtubules) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli, cytoplasm & cytoskeleton (microtubules).
 

Summary

Staining of cytoplasm and nucleus in the three cell lines with additional staining of cytoskeleton (microtubules) in U-251 MG and U-2 OS.  

Validation

The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Microtubules
Nucleus but not nucleoli