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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Vesicles 

Staining summary

Mainly localized to the nucleus but also localized to vesicles in A-431 cells.

Reliability (APE)

Medium

Antibodies in assay

CAB005034, CAB018387, HPA023887
 

Antibody CAB005034

 

Antibody CAB018387

 

Antibody HPA023887

 

         Cell line A-431

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Location(s)

Vesicles Nucleus but not nucleoli, Vesicles  

Staining characteristics

   

Staining intensity

Strong Moderate Negative 

Validation

Location not consistent with experimental gene/protein characterization data.
 
One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 
No staining.
 

Comment

   

         Cell line U-2 OS

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Location(s)

 Nucleus but not nucleoli   

Staining characteristics

    

Staining intensity

Negative Moderate   

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
   

Comment

    

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli  

Staining characteristics

   

Staining intensity

Negative Moderate Negative 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
No staining.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in vesicles.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-251MG shows no positivity.
 

Summary

Staining of vesicles in A-431.   Staining of the nucleus in all three cell lines. Additional staining of vesicles in A-431.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is not consistent with literature.
 
The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Nucleus but not nucleoli
Endosome