Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

Cytoplasm 

Staining summary

Mainly localized to the nucleus but also present in the cytoplasm.

Reliability (APE)

Very low

Antibodies in assay

CAB002418, HPA024353
 

Antibody CAB002418

 

Antibody HPA024353

 

         Cell line A-431

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Location(s)

 Nucleus, Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Weak 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Strong 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

No staining was observed in any of the three cell lines.   Staining of nucleus in all three cell lines with additional staining of cytoplasm in U-251 MG and A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Nucleus