Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Cytoplasm

Additional location(s)

Mitochondria 

Staining summary

Mainly localized to the cytoplasm and nucleus but also to mitochondria.

Reliability (APE)

Medium

Antibodies in assay

HPA002044, HPA005471
 

Antibody HPA002044

 

Antibody HPA005471

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm   

Staining characteristics

Granular   

Staining intensity

Weak   

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
   

Comment

   

         Cell line U-2 OS

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Location(s)

Cytoplasm Cytoplasm, Mitochondria 

Staining characteristics

Granular  

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus, Cytoplasm, Mitochondria 

Staining characteristics

Granular  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & mitochondria.
 

Summary

Staining of cytoplasm and/or nucleus in all three cell lines.   Staining of mitochondria and cytoplasm in U-251 MG and U-2 OS with additional staining of nucleus in U-251 MG.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Mitochondria
Nucleus