The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human bone marrow shows distinct cytoplasmic positivity in bone marrow poietic cells.
Immunohistochemical staining of human corpus, uterine shows distinct cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
Summary
Renal tubules and megakaryocytes in bone marrow displayed strong cytoplasmic immunoreactivity while the intestine, trophoblasts and cilia in e.g. respiratory epithelium and fallopian tubes showed distinct membranous staining. Moderate staining was observed in hepatocytes, decidual cells and smooth muscle. Remaining normal tissues showed weak cytoplasmic positivity or were negative.
Strong cytoplasmic and occasional nuclear positivity was seen in cerebellum, glandular cells in female genitalia as well as subsets of cells in seminiferus ducts, decidua, exocrine pancreas and neurons. Remaining normal tissues were negative.
Most of the normal tissues displayed moderate cytoplasmic and occasional nuclear positivity. Seminiferous duct cells showed distinct nuclear positivity while the thyroid gland exhibited strong cytoplasmic staining. The liver, pancreas, glial cells and lymphoid cells were weakly stained or negative.
Validation
Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
