Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Plasma membrane 

Staining summary

Mainly localized to the cytoplasm but also localized to the plasma membrane in A-431 cells.

Reliability (APE)

Medium

Antibodies in assay

HPA001040, HPA001391, CAB020817
 

Antibody HPA001040

 

Antibody HPA001391

 

Antibody CAB020817

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm    

Staining characteristics

Granular    

Staining intensity

Weak Negative   

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
No staining.
   

Comment

    

         Cell line U-2 OS

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Location(s)

Cytoplasm   

Staining characteristics

Granular   

Staining intensity

Weak Negative Negative 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
No staining.
 
No staining.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Cytoplasm   

Staining characteristics

Granular   

Staining intensity

Weak Negative Negative 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
No staining.
 
No staining.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows no positivity.
 

Immunofluorescent staining of human cell line U-251 MG shows no positivity.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of plasma membrane in A-431.   No staining was observed in any of the three cell lines.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Plasma membrane