Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Mitochondria

Additional location(s)

Cytoplasm 

Staining summary

Staining of nucleus and mitochondria in all three cell lines with additional staining of cytoplasm in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA035860
 

Antibody HPA035860

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Mitochondria 

Staining characteristics

 

Staining intensity

Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Mitochondria 

Staining characteristics

 

Staining intensity

Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Mitochondria 

Staining characteristics

 

Staining intensity

Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & mitochondria.
 

Summary

Staining of nucleus and mitochondria in all three cell lines with additional staining of cytoplasm in U-2 OS.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Mitochondria
Nucleus but not nucleoli