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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

Nucleus but not nucleoli, Focal Adhesions 

Staining summary

Mainly localized to the cytoplasm and plasma membrane but also to the nucleus and focal adhesion sites.

Reliability (APE)

Medium

Antibodies in assay

HPA023696, CAB040554
 

Antibody HPA023696

 

Antibody CAB040554

 

         Cell line A-431

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Location(s)

  Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

   

Staining intensity

  Moderate 

Validation

  
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm Plasma membrane, Cytoplasm, Focal Adhesions 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli, plasma membrane & cytoplasm.
 

Summary

Staining of cytoplasm and nuclei in all three cell lines.   Staining of plasma membrane and cytoplasm in all three cell lines. Additional staining of nucleus in U-251 MG and A-431. Additional staining of focal adhesions in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Focal adhesions
Nucleus but not nucleoli
Plasma membrane