Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Plasma membrane

Additional location(s)

Golgi apparatus 

Staining summary

Mainly localized to the plasma membrane but also to the Golgi apparatus.

Reliability (APE)

High

Antibodies in assay

CAB000112, CAB000316, HPA005785
 

Antibody CAB000112

 

Antibody CAB000316

 

Antibody HPA005785

 

         Cell line A-431

Toggle channels

 
 
 

Location(s)

Plasma membrane Plasma membrane, Golgi apparatus Cytoplasm, Golgi apparatus 

Staining characteristics

  Granular 

Staining intensity

Moderate Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

Toggle channels

  
 
 

Location(s)

  Plasma membrane, Golgi apparatus Plasma membrane 

Staining characteristics

    

Staining intensity

  Moderate Moderate 

Validation

  
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

    

         Cell line U-251 MG

Toggle channels

 
 
 

Location(s)

 Plasma membrane, Golgi apparatus Plasma membrane 

Staining characteristics

   

Staining intensity

Negative Moderate Strong 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane.
 

Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & golgi apparatus.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane.
 

Summary

Staining of plasma membrane in A-431.   Staining of plasma membrane in all three cell lines with additional staining of Golgi.   Staining of plasma membrane in U-251MG and U-2 OS. Staining of Golgi apparatus and cytoplasm in A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Golgi apparatus
Plasma membrane