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SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleoli, Cytoskeleton (Actin filaments), Centrosome 

Staining summary

Localized to the cytoplasm, the centrosome and actin filaments.

Reliability (APE)

Low

Antibodies in assay

CAB004549, HPA019669, HPA024235
 

Antibody CAB004549

 

Antibody HPA019669

 

Antibody HPA024235

 

         Cell line A-431

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Location(s)

 Nucleoli, Cytoplasm Centrosome 

Staining characteristics

   

Staining intensity

Negative Moderate Weak 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

 Nucleoli, Cytoplasm, Cytoskeleton (Actin filaments) Cytoplasm, Centrosome 

Staining characteristics

   

Staining intensity

Negative Strong Weak 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

 Nucleoli, Cytoplasm Centrosome 

Staining characteristics

   

Staining intensity

Negative Strong Weak 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli, cytoplasm & cytoskeleton (actin filaments).
 

Immunofluorescent staining of human cell line U-251MG shows positivity in centrosome.
 

Summary

No staining was observed in any of the three cell lines.   Staining of nucleoli and cytoplasm in all three cell lines with additional staining of cytoskeleton (actin filaments) in U-2 OS.   Staining of centrosome in all three cell lines, with additional staining of cytoplasm in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Cytoplasm
Actin filaments
Nucleoli