The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human cerebellum shows distinct cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemical staining of human stomach shows moderate positivity in chief cells.
Summary
Most normal cells showed moderate to strong cytoplasmic immunoreactivity. Smooth muscle cells and most hepatocytes were negative.
Normal tissues generally displayed weak to moderate cytoplasmic positivity. Purkinje cells, renal tubules and pancreatic islets displayed strong cytoplasmic positivity. Cells in the CNS, male genital tract and squamous epithelia were weakly stained or negative.
Most normal tissues displayed weak to moderate cytoplasmic positivity. Strong staining was observed in exocrine pancreas and adrenal cortical cells. Bile duct cells, renal glomeruli, ovarian and endometrial stroma cells were negative.
Exocrine pancreas displayed strong cytoplasmic staining. Gastrointestinal tract, islet cells, seminal vesicle, trophoblastic cells, salivary glands and some squamous epithelium exhibited moderate cytoplasmic immunoreactivity. Remaining normal cells were weakly stained or negative.
Validation
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
