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SUBCELLULAR LOCATION

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Main location(s)

Nucleoli, Nucleus but not nucleoli, Cytoplasm

Additional location(s)

Mitochondria 

Staining summary

Localized to the cytoplasm, nucleus or mitochondria.

Reliability (APE)

Very low

Antibodies in assay

HPA020091, CAB020682, HPA021815, HPA030388
 

Antibody HPA020091

 

Antibody CAB020682

 

Antibody HPA021815

 

Antibody HPA030388

 

         Cell line A-431

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Location(s)

Cytoplasm Cytoplasm Nucleoli, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

  Cluster  

Staining intensity

Moderate Moderate Strong Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

    

         Cell line U-2 OS

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Location(s)

Cytoplasm, Mitochondria Cytoplasm Nucleoli, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

  Cluster  

Staining intensity

Moderate Weak Strong Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

    

         Cell line U-251 MG

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Location(s)

Cytoplasm Cytoplasm Nucleoli, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

Granular, Smooth  Cluster  

Staining intensity

Moderate Weak Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

    

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of mitochondria in U-2 OS.   Staining of cytoplasm in all three cell lines.   Staining of nucleoli and cytoplasm in all three cell lines.   Staining of the nucleus in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Mitochondria
Nucleoli
Nucleus but not nucleoli