Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Vesicles, Cytoskeleton (Microtubules)

Additional location(s)

Cytoskeleton (Intermediate filaments) 

Staining summary

Localized to the nucleus, vesilces and microtubules.

Reliability (APE)

Very low

Antibodies in assay

HPA031415, HPA031417
 

Antibody HPA031415

 

Antibody HPA031417

 

         Cell line A-431

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Location(s)

Vesicles, Cytoskeleton (Intermediate filaments), Cytoskeleton (Microtubules) Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Strong Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Vesicles, Cytoskeleton (Microtubules) Nucleus, Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Vesicles, Cytoskeleton (Microtubules) Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Strong Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in vesicles & cytoskeleton (microtubules).
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & cytoplasm.
 

Summary

Staining of vesicles and microtubules in all three cell lines with additional staining of intermediate filaments in A-431.   Staining of nucleus in all three cell lines. Additional staining of cytoplasm in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 

Dictionary

Intermediate filaments
Microtubules
Nucleus
Endosome