Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

Nucleoli, Cytoplasm 

Staining summary

The protein is mainly localized to the nucleus. Additionally localized to the cytoplasm and nucleoli.

Reliability (APE)

Low

Antibodies in assay

HPA018843, HPA019080, HPA019092
 

Antibody HPA018843

 

Antibody HPA019080

 

Antibody HPA019092

 

         Cell line A-431

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Location(s)

Nucleus, Nucleoli Nucleus Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

   

Staining intensity

Strong Moderate Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus, Nucleoli Nucleus Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Spotty  Granular 

Staining intensity

Strong Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleoli, Nuclear membrane, Cytoplasm Nucleus Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Spotty  Granular 

Staining intensity

Strong Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus & nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of nucleus and nucleoli in all three cell lines with additional staining of cytoplasm in U-251 MG.   Staining of nuclei in a fraction of cells for all three cell lines.   Staining of nucleus and cytoplasm in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleoli
Nucleus