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SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm, Cytoskeleton (Intermediate filaments), Centrosome

Additional location(s)

 

Staining summary

Localized to the cytoplasm, centrosomes or intermediate filaments.

Reliability (APE)

Very low

Antibodies in assay

HPA037376, HPA037565
 

Antibody HPA037376

 

Antibody HPA037565

 

         Cell line A-431

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Location(s)

Cytoplasm, Centrosome Cytoskeleton (Intermediate filaments) 

Staining characteristics

  

Staining intensity

Strong Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm, Centrosome Aggresome 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Centrosome  

Staining characteristics

  

Staining intensity

Strong Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
No staining.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in centrosome.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in aggresome.
 

Summary

Staining of centrosome in all three cell lines and of cytoplasm in A-431 and U-2 OS.   Staining of aggresome in U-251 MG and of cytoskeleton (intermediate filaments) in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Cytoplasm
Intermediate filaments