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SUBCELLULAR LOCATION

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Main location(s)

Mitochondria

Additional location(s)

Cytoskeleton (Actin filaments) 

Staining summary

Localized to the mitochondria and actin filaments.

Reliability (APE)

Low

Antibodies in assay

HPA029133, HPA029134
 

Antibody HPA029133

 

Antibody HPA029134

 

         Cell line A-431

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Location(s)

Cytoskeleton (Actin filaments)   

Staining characteristics

   

Staining intensity

Weak   

Validation

Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
   

Comment

   

         Cell line U-2 OS

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Location(s)

Mitochondria, Cytoskeleton (Actin filaments) Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Mitochondria, Cytoskeleton (Actin filaments) Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria & cytoskeleton (actin filaments).
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Summary

Staining of cytoskeleton (actin filaments) in A-431. Staining of cytoskeleton (actin filaments) and mitochondria in U-251 MG and U-2 OS.   Staining of cytoplasm in U-2 OS and U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 

Dictionary

Actin filaments
Mitochondria