Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the plasma membrane and cytoplasm in the three cell lines.

Reliability (APE)

High

Antibodies in assay

HPA020980, HPA029504
 

Antibody HPA020980

 

Antibody HPA029504

 

         Cell line A-431

Toggle channels

 
 

Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

Toggle channels

 
 

Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Strong Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

Toggle channels

 
 

Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Strong Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of cytoplasm and plasma membrane in all three cell lines.   Staining of cytoplasm and plasma membrane in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Plasma membrane