AKR7A2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody CAB032587

 

Antibody CAB032841

 

ANTIBODY INFORMATION

Provider

Santa Cruz Biotechnology
 Santa Cruz Biotechnology
 

Product name

sc-27766 sc-32944 

Host species

Goat Rabbit 

Clonality

msAb pAb 

Purity

Affinity Protein A/G 

Released in version

12 7 

ANTIGEN INFORMATION

Antigen

Synthetic peptide Recombinant protein 

Length (aa)

  

Antigen sequence

 
 

Matching transcripts

  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
More information
 Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:10 1:75 

Validation IH

Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

39.6, 34.7, 21.6, 6.1 39.6, 34.7, 21.6, 6.1 

Antibody dilution

1:500 1:500 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Immunofluorescence

Image

  

Description

 Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.
More information
 

Antibody dilution

 1:19 

Validation IF

 Supportive: The subcellular location is supported by literature. 
 

ANTIGEN VIEW

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 Not available