Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Plasma membrane, Cytoskeleton (Intermediate filaments) 

Staining summary

Mainly localized to nuclear speckles.

Reliability (APE)

Medium

Antibodies in assay

HPA026439, HPA028490, HPA028526
 

Antibody HPA026439

 

Antibody HPA028490

 

Antibody HPA028526

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus Nucleus but not nucleoli, Plasma membrane 

Staining characteristics

Speckle Speckle Speckle 

Staining intensity

Weak Weak Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoskeleton (Intermediate filaments) Nucleus Nucleus but not nucleoli, Plasma membrane 

Staining characteristics

 Speckle Speckle 

Staining intensity

Strong Weak Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Cytoskeleton (Intermediate filaments) Nucleus Plasma membrane 

Staining characteristics

 Speckle  

Staining intensity

Strong Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoskeleton (intermediate filaments).
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & plasma membrane.
 

Summary

Staining of nucleus and/or intermediate filaments.   Staining of nucleus in all three cell lines.   Staining of the plasma membrane in U-251 MG. Staining of the plasma membrane and nucleus in A-431 and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Intermediate filaments
Nucleus but not nucleoli
Plasma membrane