Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus but not nucleoli, Centrosome 

Staining summary

Mainly localized to the cytoplasm. In addition localized ti centrosome and nucleus.

Reliability (APE)

Very low

Antibodies in assay

HPA001767, CAB002487
 

Antibody HPA001767

 

Antibody CAB002487

 

         Cell line A-431

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Location(s)

Cytoplasm, Centrosome  

Staining characteristics

Granular  

Staining intensity

Moderate Negative 

Validation

Location not consistent with experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm  

Staining characteristics

Granular  

Staining intensity

Moderate Negative 

Validation

Location not consistent with experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Centrosome  

Staining characteristics

  

Staining intensity

Weak Negative 

Validation

Location not consistent with experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Summary

Staining of cytoplasm in all three cell lines. Additional staining of centrosomes in A-431 and nuclei and centrosomes in U-251 MG.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is not consistent with literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Cytoplasm
Nucleus but not nucleoli