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SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus but not nucleoli, Mitochondria, Centrosome 

Staining summary

Mainly localized to cytoplasm. Additionally localized to nucleus, mitochondria and centrosome.

Reliability (APE)

Low

Antibodies in assay

HPA001302, CAB002047, HPA005688
 

Antibody HPA001302

 

Antibody CAB002047

 

Antibody HPA005688

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm  Cytoplasm, Mitochondria, Centrosome 

Staining characteristics

Granular   

Staining intensity

Moderate Negative Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
No staining.
 
One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm   Mitochondria 

Staining characteristics

Granular    

Staining intensity

Moderate   Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
   
One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 

Comment

    

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm  Cytoplasm, Mitochondria, Centrosome 

Staining characteristics

Granular  Granular 

Staining intensity

Moderate Negative Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
No staining.
 
One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria.
 

Summary

Staining of nucleus and cytoplasm in all three cell lines.   No staining was observed in any of the three cell lines.   Staining of mitochondria in all three cell lines. Additional staining of cytoplasm and centrosomes in U-251MG and A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Centrosome
Cytoplasm
Mitochondria
Nucleus but not nucleoli