Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus

Additional location(s)

Nucleoli, Plasma membrane, Cytoplasm, Golgi apparatus 

Staining summary

Mainly localized to the nucleus. In addition detected in nucleoli, the Golgi apparatus, plasma membrane and cytoplasm.

Reliability (APE)

Very low

Antibodies in assay

HPA030353, HPA030354, HPA030355, HPA030356
 

Antibody HPA030353

 

Antibody HPA030354

 

Antibody HPA030355

 

Antibody HPA030356

 

         Cell line A-431

Toggle channels

 
 
 
 

Location(s)

 Nucleoli Nucleus but not nucleoli, Golgi apparatus Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

  Granular  

Staining intensity

Negative Moderate Moderate Moderate 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

    

         Cell line U-2 OS

Toggle channels

 
 
 
 

Location(s)

 Nucleoli Nucleus, Nucleoli, Golgi apparatus Plasma membrane 

Staining characteristics

    

Staining intensity

Negative Moderate Weak Strong 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

    

         Cell line U-251 MG

Toggle channels

 
 
 
 

Location(s)

 Nucleoli Nucleoli, Nucleus but not nucleoli, Golgi apparatus Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

  Spotty Spotty 

Staining intensity

Negative Moderate Weak Moderate 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

    

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleoli.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleoli, nucleus but not nucleoli & golgi apparatus.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane.
 

Summary

No staining was observed in amy of the three cell lines.   Staining of nucleoli in all three cell lines.   Staining of nucleus and Golgi apparatus in all three cell lines with additional staining of nucleoli in U-251 MG and U-2 OS.   Staining of plasma membrane in all three cell lines with additional staining of nucleus and cytoplasm in U-251 MG and A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Golgi apparatus
Nucleoli
Nucleus
Plasma membrane