Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Plasma membrane 

Staining summary

Mainly localized to the cytoplasm.

Reliability (APE)

High

Antibodies in assay

CAB019421, HPA029501
 

Antibody CAB019421

 

Antibody HPA029501

 

         Cell line A-431

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Location(s)

Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines.   Staining of cytoplasm in all three cell lines, with additional staining of plasma membrane in A-431 and U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Plasma membrane