Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Mitochondria

Additional location(s)

Nucleoli, Nucleus but not nucleoli 

Staining summary

Mainly localized to mitochondria but also to the nucleus.

Reliability (APE)

High

Antibodies in assay

HPA000425, HPA002017
 

Antibody HPA000425

 

Antibody HPA002017

 

         Cell line A-431

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli, Mitochondria Nucleoli, Mitochondria 

Staining characteristics

Granular Smooth 

Staining intensity

Weak Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli, Mitochondria Nucleoli, Mitochondria 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli, Mitochondria Nucleoli, Mitochondria 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & mitochondria.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & mitochondria.
 

Summary

Mitochondrial staining in U-251MG and U2-OS. Additional weaker staining of nucleus in all three cell lines.   Staining of mitochondria and nucleoli in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Mitochondria
Nucleoli
Nucleus but not nucleoli