The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Cytoplasmic and membranous expression several cell types, most abundant in testicular Leydig cells, placental trophoblasts, renal glomeruli and basal cells of squamous epithelium.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells and weak staining in cells of seminiferus ducts.
Immunohistochemical staining of human kidney shows distinct membranous positivity in cells in glomeruli.
Summary
Strong membranous staining was observed in trophoblastic and Leydig cells. Gall bladder showed strong granular cytoplasmic staining. Basal cells of squamous epithelia, cells in CNS, pancreatic islets, renal glomeruli and breast showed moderate cytoplasmic positivity with additional membranous staining in several cases. were distinctly positive. Most other normal cells were weakly stained or negative.
Most normal tissues displayed moderate cytoplasmic immunoreactivity. Renal glomeruli, Leydig cells and trophoblastic cells exhibited distinct membranous staining. Lymphoid tissue was negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
