Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus but not nucleoli 

Staining summary

Mainly localized to the cytoplasm. In addition localized to the nucleus.

Reliability (APE)

Low

Antibodies in assay

CAB005184, HPA030262
 

Antibody CAB005184

 

Antibody HPA030262

 

         Cell line A-431

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Location(s)

 Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Strong 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Cytoplasm, Cytoskeleton (Microtubules) 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm Cytoplasm 

Staining characteristics

 Fibrous 

Staining intensity

Moderate Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & cytoskeleton (microtubules).
 

Summary

Staining of cytoplasm in U-251 MG and nucleus in U-2 OS.   Staining of cytoplasm in all three cell lines with additional staining of cytoskeleton (microtubules) in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli