CAMK2A

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA051783

 

Antibody HPA051785

 

Antibody HPA053973

 

Antibody CAB004330

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Santa Cruz Biotechnology
 

Product name

HPA051783 HPA051785 HPA053973 sc-5306 

Host species

Rabbit Rabbit Rabbit Mouse 

Clonality

pAb pAb pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

12 12 12 2 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment Recombinant protein 

Length (aa)

26 27 33  

Antigen sequence

ELFEDIVAREYYSEADASHCIQQILE
 
LYQQIKAGAYDFPSPEWDTVTPEAKDL
 
GQEYAAKIINTKKLSARDHQKLEREARICRLLK
 
 

Matching transcripts

CAMK2A-001 - ENSP00000261793 [100%]
CAMK2A-002 - ENSP00000381412 [100%]
 CAMK2A-001 - ENSP00000261793 [100%]
CAMK2A-002 - ENSP00000381412 [100%]
 CAMK2A-001 - ENSP00000261793 [100%]
CAMK2A-002 - ENSP00000381412 [100%]
CAMK2A-005 - ENSP00000426607 [100%]
  

Other gene match

CAMK2B - ENSG00000058404 [100%]
CAMK2D - ENSG00000145349 [100%]
CAMK2G - ENSG00000148660 [96%]
 CAMK2B - ENSG00000058404 [96%]
CAMK2D - ENSG00000145349 [100%]
CAMK2G - ENSG00000148660 [96%]
 CAMK2B - ENSG00000058404 [97%]
CAMK2D - ENSG00000145349 [100%]
CAMK2G - ENSG00000148660 [97%]
  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

    

Description

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
More information
 Immunohistochemical staining of human hippocampus shows moderate positivity in subsets of neuronal cells.
More information
 Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
More information
 Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:275 1:10 1:175 1:150 

Validation IH

Supportive: The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for all of the genes Uncertain: The multi-targeting antibody yielding a staining pattern partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data for all of the genes Supportive: The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for all of the genes Uncertain: One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Western Blot

Image

    

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: U-251 MG
Lane 3: Human Plasma
Lane 4: Liver
Lane 5: Tonsil
Lane 6: RT-4
More information
 

Target mass (kDa)

72.7, 65.2, 62.2, 60.4, 60.0, 59.8, 59.6, 59.2, 59.0, 58.5, 58.0, 57.9, 57.8, 57.6, 56.4, 56.3, 56.0, 55.7, 55.4, 55.3, 55.2, 54.1, 51.0, 50.9, 41.1, 20.5, 19.6 72.7, 65.2, 62.2, 60.4, 60.0, 59.8, 59.6, 59.2, 59.0, 58.5, 58.0, 57.9, 57.8, 57.6, 56.4, 56.3, 56.0, 55.7, 55.4, 55.3, 55.2, 54.1, 51.0, 50.9, 41.1, 36.9, 33.3, 22.1 72.7, 65.2, 62.2, 60.4, 60.0, 59.8, 59.6, 59.2, 59.0, 58.5, 58.0, 57.9, 57.8, 57.6, 56.4, 56.3, 56.0, 55.7, 55.4, 55.3, 55.2, 54.1, 51.0, 41.1, 20.5, 19.6, 19.0, 12.8, 10.6 55.3, 54.1, 10.6, 2.6 

Antibody dilution

1:250 1:110 1:150 1:500 

Validation WB

Uncertain: Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data 

         Protein array

Image

    

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
  

Antibody dilution

1:2000 1:900 1:1200  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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CAMK2A-001
 
CAMK2A-002
 
CAMK2A-005