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SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Cytoplasm

Additional location(s)

Nucleoli, Plasma membrane 

Staining summary

Localized to nucleus or cytoplasm.

Reliability (APE)

Very low

Antibodies in assay

CAB005605, HPA011166, HPA012945
 

Antibody CAB005605

 

Antibody HPA011166

 

Antibody HPA012945

 

         Cell line A-431

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Location(s)

Nucleoli, Cytoplasm   Nucleus 

Staining characteristics

   Granular 

Staining intensity

Weak   Weak 

Validation

Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
   
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

    

         Cell line U-2 OS

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Location(s)

Nucleoli, Cytoplasm  Nucleus 

Staining characteristics

   

Staining intensity

Moderate Negative Weak 

Validation

Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 
No staining.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleoli, Plasma membrane  Nucleus but not nucleoli 

Staining characteristics

   

Staining intensity

Weak Negative Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
No staining.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleoli & plasma membrane.
 

Immunofluorescent staining of human cell line U-251MG shows no positivity.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nucleus in all three cell lines. Staining of cytoplasm in A-431 and U-2 OS and of plasma membrane in U-251 MG.   No staining was observed in any of the three cell lines.   Staining of nucleus in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Nucleoli
Nucleus
Plasma membrane