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SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Mitochondria

Additional location(s)

Nucleoli, Cytoplasm 

Staining summary

Mainly localized to mitochondria and nucleus but also nucleoli and cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

HPA002852, HPA003904
 

Antibody HPA002852

 

Antibody HPA003904

 

         Cell line A-431

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Location(s)

Nucleus, Nucleoli, Cytoplasm, Mitochondria Nucleus but not nucleoli, Cytoplasm, Mitochondria 

Staining characteristics

Granular Granular 

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus, Nucleoli, Mitochondria Nucleus but not nucleoli, Mitochondria 

Staining characteristics

Smooth Smooth 

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus, Cytoplasm, Mitochondria Nucleus but not nucleoli, Cytoplasm, Mitochondria 

Staining characteristics

Granular Granular 

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus, nucleoli & mitochondria.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & mitochondria.
 

Summary

Staining of nucleus and mitochondria in all three cell lines. Additional staining of cytoplasm in U-251 MG, nucleoli in U-2 OS and both cytoplasm and nucleoli in A-431.   Staining of nucleus and mitochondria in all three cell lines. Additional staining of cytoplasm in U-251MG and A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Mitochondria
Nucleoli
Nucleus