Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Plasma membrane

Additional location(s)

Nucleus but not nucleoli, Cytoplasm, Focal Adhesions 

Staining summary

Staining of plasma membrane/cytoplasm in all three cell lines with additional staining of focal adhesion sites in U-251 MG and nucleus in A-431.

Reliability (Single)

Antibodies in assay

HPA019035
 

Antibody HPA019035

 

         Cell line A-431

Toggle channels

 

Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-2 OS

Toggle channels

 

Location(s)

Plasma membrane 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

Toggle channels

 

Location(s)

Plasma membrane, Cytoplasm, Focal Adhesions 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane.
 

Summary

Staining of plasma membrane/cytoplasm in all three cell lines with additional staining of focal adhesion sites in U-251 MG and nucleus in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Focal adhesions
Nucleus but not nucleoli
Plasma membrane