Fields »

SUBCELLULAR LOCATION

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Main location(s)

Golgi apparatus

Additional location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining summary

Mainly localized to the Golgi apparatus.

Reliability (APE)

Medium

Antibodies in assay

CAB002681, HPA035876
 

Antibody CAB002681

 

Antibody HPA035876

 

         Cell line A-431

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Location(s)

  Cytoplasm, Golgi apparatus 

Staining characteristics

   

Staining intensity

  Strong 

Validation

  
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

  Cytoplasm, Golgi apparatus 

Staining characteristics

   

Staining intensity

  Moderate 

Validation

  
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli, Cytoplasm, Golgi apparatus 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows no positivity.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm & golgi apparatus.
 

Summary

No staining was observed in any of the three cell lines.   Staining of cytoplasm and Golgi apparatus in all three cell lines with additional staining of nucleus in U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Golgi apparatus
Nucleus but not nucleoli