Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Cytoplasm 

Staining summary

Mainly localized to the nucleus but also to the cytoplasm.

Reliability (APE)

Low

Antibodies in assay

HPA014967, HPA018107
 

Antibody HPA014967

 

Antibody HPA018107

 

         Cell line A-431

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Location(s)

  Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  Granular, Spotty 

Staining intensity

  Moderate 

Validation

  
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Cell line U-2 OS

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Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-251 MG

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Location(s)

  Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  Spotty 

Staining intensity

  Weak 

Validation

  
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

No staining was observed in any of the three cell lines.   Staining of cytoplasm and nucleus in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli