KCNQ2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA016642

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA016642 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

137 

Antigen sequence

FYATNLSRTDLHSTWQYYERTVTVPMYRLIPPLNQLELLRNLKSKSGLAF
RKDPPPEPSPSQKVSLKDRVFSSPRGVAAKGKGSPQAQTVRRSPSADQSL
EDSPSKVPKSWSFGDRSRARQAFRIKGAASRQNSEEA
 

Matching transcripts

KCNQ2-005 - ENSP00000359242 [100%]
KCNQ2-007 - ENSP00000359244 [100%]
KCNQ2-011 - ENSP00000353668 [100%]
KCNQ2-201 - ENSP00000346601 [100%]
KCNQ2-001 - ENSP00000349789 [93%]
KCNQ2-002 - ENSP00000339611 [91%]
KCNQ2-004 - ENSP00000359246 [85%]
KCNQ2-203 - ENSP00000399612 [84%]
KCNQ2-003 - ENSP00000352035 [83%]
KCNQ2-202 - ENSP00000352718 [83%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje and molecular layer cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:50 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

97.1, 97.0, 95.8, 95.7, 94.1, 93.1, 92.7, 92.6, 68.2 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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KCNQ2-001
 
KCNQ2-002
 
KCNQ2-003
 
KCNQ2-004
 
KCNQ2-005
 
KCNQ2-007
 
KCNQ2-011
 
KCNQ2-201
 
KCNQ2-202
 
KCNQ2-203