The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
General cytoplasmic expression in essentially all cells except in lymphoid cells, glial cells and stromal cells.
Caution: Based on antibodies targeting proteins from multiple genes.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Summary
Strong cytoplasmic and membranous staining was observed mainly in glandular epithelia and neuronal cells. Other normal tissues generally displayed moderate positivity, in squamous epithelia only in basal cell layers. Endothelial cells showed distinct immunoreactivity.
Strong cytoplasmic immunoreactivity was observed in neuronal cells, gall bladder, thyroid glands, heart and skeletal muscle. Distinct staining was also observed in neuropil. Lymphoid cells were negative. Other normal tissues generally showed weak to moderate cytoplasmic positivity.
Validation
The multi-targeting antibody yielding a staining pattern partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data for all of the genes
One antibody yielding a staining pattern which is consistent with gene/protein characterization data
