N4BP2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA036770

 

Antibody HPA042607

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA036770 HPA042607 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

90 88 

Antigen sequence

FSLGLNLKEIISQRGTLENSNSPVPEFSHGIGISNADSQSTCDAERGNSE
QAEMRAVTPENHESMTSIFPSAAVGLKNNNDILPNSQEEL
 
PSSDSLAQREHRSRMPKTGLSEPNLEIGTNDKMNEISLSTAHEACWGTSS
QKLKTLGSSNLGSSEMLLSEMTCESQTCLSKKSHGQHT
 

Matching transcripts

N4BP2-001 - ENSP00000261435 [100%]
N4BP2-003 - ENSP00000426430 [100%]
 N4BP2-001 - ENSP00000261435 [100%]
N4BP2-003 - ENSP00000426430 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
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Retrieval method

HIER pH6  

Antibody dilution

1:30  

Validation IH

Uncertain: One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data  

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & centrosome.
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 Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane, cytoplasm & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:31 1:122 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

  

Description

  

Target mass (kDa)

198.8, 158.1 198.8, 158.1 

Antibody dilution

1:250 1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:12000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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N4BP2-001
 
N4BP2-003