DNM2

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA054246

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA054246 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

11 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

23 

Antigen sequence

PPQIPSRPVRIPPGIPPGVPSRR
 

Matching transcripts

DNM2-001 - ENSP00000347890 [100%]
DNM2-002 - ENSP00000352721 [100%]
DNM2-003 - ENSP00000386192 [100%]
DNM2-010 - ENSP00000373905 [100%]
DNM2-201 - ENSP00000313164 [100%]
DNM2-006 - ENSP00000468734 [91%]
DNM2-023 - ENSP00000466914 [87%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:350 

Validation IH

Supportive: One antibody yielding a staining pattern which is consistent with gene/protein characterization data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line HeLa shows positivity in Golgi apparatus.
More information
 

Antibody dilution

1:8 

Validation IF

Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

98.1, 98.0, 97.7, 97.6, 14.3 

Antibody dilution

1:80 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:650 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

? »
 
 
 
DNM2-001
 
DNM2-002
 
DNM2-003
 
DNM2-006
 
DNM2-010
 
DNM2-023
 
DNM2-201