Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Plasma membrane

Additional location(s)

Nucleus, Cell Junctions 

Staining summary

Mainly localized to the plasma membrane and cell junctions. In U-251 MG cells the protein is also expressed in the nucleus.

Reliability (APE)

High

Antibodies in assay

HPA005730, HPA006642
 

Antibody HPA005730

 

Antibody HPA006642

 

         Cell line A-431

Toggle channels

 
 

Location(s)

Plasma membrane Plasma membrane 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

Toggle channels

 
 

Location(s)

Plasma membrane, Cell Junctions Plasma membrane, Cell Junctions 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli Nucleus, Plasma membrane 

Staining characteristics

Granular  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cell junctions.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cell junctions.
 

Summary

Staining of plasma membrane in A-431 and U-2 OS. Staining of nucleus in U-251MG.   Staining of plasma membrane in all three cell lines. Additional staining of nucleus in U-251 MG and cell junctions in U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cell junctions
Nucleus
Plasma membrane