The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in melanocytes.
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in melanocytes.
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in melanocytes.
Summary
Melanocytes showed strong cytoplasmic immunoreactivity. Positivity was also observed in the thyroid, alveolar macrophages, subsets of cells in the CNS and testis. All other cells were negative.
Melanocytes in skin showed strong cytoplasmic staining while a subset of cells in bone marrow displayed moderate cytoplasmic positivity. Remaining normal cells were negative.
Melanocytes in skin exhibited strong cytoplasmic staining. Positivity was also observed in Leydig cells of testis and neurons in CNS. Remaining normal cells were generally were negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
