Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus but not nucleoli

Additional location(s)

Vesicles 

Staining summary

Staining of nucleus in all three cell lines with additional staining of vesicles in A-431 and U-2 OS.

Reliability (Single)

Antibodies in assay

HPA004751
 

Antibody HPA004751

 

         Cell line A-431

Toggle channels

 

Location(s)

Nucleus but not nucleoli, Vesicles 

Staining characteristics

Speckle 

Staining intensity

Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

Toggle channels

 

Location(s)

Nucleus but not nucleoli, Vesicles 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

Toggle channels

 

Location(s)

Nucleus but not nucleoli 

Staining characteristics

Speckle 

Staining intensity

Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nucleus in all three cell lines with additional staining of vesicles in A-431 and U-2 OS.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Nucleus but not nucleoli
Endosome