Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Cytoplasm

Additional location(s)

 

Staining summary

Speckled staining of nucleus and staining of cytoplasm in all three cell lines.

Reliability (Single)

Antibodies in assay

HPA008709
 

Antibody HPA008709

 

         Cell line A-431

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Location(s)

Nucleus, Cytoplasm 

Staining characteristics

Speckle 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus, Cytoplasm 

Staining characteristics

Granular, Speckle 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Speckle 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & cytoplasm.
 

Summary

Speckled staining of nucleus and staining of cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus