CAD

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA050746

 

Antibody HPA057266

 

Antibody CAB007781

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Epitomics
 

Product name

HPA050746 HPA057266 1697-1 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Supernatant 

Released in version

13 13 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Synthetic peptide 

Length (aa)

120 82  

Antigen sequence

FSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVI
NAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACL
LTQYRVSLRYVAPPSLRMPP
 
VLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNE
ISVEVDSDPRAAYFRQAENGMYIRMALLATVL
 
 

Matching transcripts

CAD-001 - ENSP00000264705 [100%]
CAD-002 - ENSP00000384510 [100%]
CAD-011 - ENSP00000405416 [100%]
 CAD-001 - ENSP00000264705 [100%]
CAD-002 - ENSP00000384510 [100%]
CAD-011 - ENSP00000405416 [100%]
  

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Application not done for this antibody. Application not done for this antibody. Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.
More information
 

Retrieval method

  HIER pH6 

Antibody dilution

  1:25 

Literature conformity

  Consistent with extensive gene/protein characterization data 

RNA consistency

  Mainly consistent with RNAseq data 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

243.0, 236.0, 32.2 243.0, 236.0, 32.2 243.0, 236.0, 32.2, 31.1, 28.9 

Antibody dilution

1:110 1:460 1:500 

Validation WB

Uncertain: No bands detected Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Immunofluorescence

Image

   

Description

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus.
More information
 Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli.
More information
 Application not done for this antibody. 

Antibody dilution

1:22 1:137  

Validation IF

Supportive: The subcellular location is supported by literature. Supportive: The subcellular location is supported by literature.  

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:850 1:5500  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

? »
 
 
 
CAD-001
 
CAD-002
 
CAD-011